Ovarian Tissue Cryopreservation: Users Manual

This manual details the NPC Implementation phase and can be done in parallel with or after completion of the NPC Membership Documents.  It can also serve as a reference for general fertility preservation programs. 

PDF icon NPC Checklist

PDF icon NPC Manual Front and Back Cover

Section 1. Oncofertility Guidelines (including Confidentiality Agreements)

PDF icon 01a. Oncofertility Consortium Guidelines

PDF icon 01b. Listing of Grant Support in Peer Reviewed Publications

Section 2. Establish your Team; Brand Your Center

PDF icon 02a. Establishing Your Team

PDF icon 02b. Branding Your Center

Additional Branding Resources

Section 3. Review the Equipment and Supplies List

PDF icon 03b. Setting Up Your Programmable Freezer

Section 4. FDA Registration

PDF icon 04. Updating or Establishing Your FDA Registration

Section 5. Memorial Blood Center: Infectious Disease Testing

IMPORTANT NOTE: If ovarian tissue is removed vaginally, it changes the infectious disease testing that must be done.  Tissue that comes into contact with the genitourinary tract necessitates GC and Chlamydia testing.

PDF icon 05a. Testing for Infectious Diseases: FDA-approved sites or Memorial Blood Center (MBC)

PDF icon 05b. Sample Blood Drawing Request

Section 6. Reprotech: Accounts, Logistics, and Donor Eligibility 

06b. Summary of Records

IMPORTANT NOTE: It is up to each site to determine if patients will be tested as anonymous donors.  If your site is doing donor eligibility in case the patients must use a gestational surrogate or other methods to establish pregnancy, donor eligibility requirements must be followed to treat the cryopreserved ovarian tissue as though it came from anonymous donor of leukocyte tissue and must undergo donor eligibility determination prior to shipping tissue to ReproTech. 

Microsoft Office document icon 06b1. HCTP SOR DED Not Performed.doc

Microsoft Office document icon 06b2. HCTP SOR DED Was Performed.doc

Microsoft Office document icon 06b3. HCTP SOR DED Subsequently Performed.doc

06c. ReproTech forms should be accessed via the NPC-specific URL for EACH PATIENThttp://forms.reprotech.com/npc-oncofertility-consortium.html

Section 7. Sage/Origio: Establishing an Account and Ordering Media 

PDF icon 07. Establishing an Account and Ordering Media from Sage

Section 8. Clinical Tips: Patient Flow, Tissue Transport, Surgical Removal and Pathology

This section describes typical patient flow, a discussion of how to transport the ovary from the operating room, tips for surgical removal and strategies for providing an adequate sample for examination by pathology.

PDF icon 08a. Typical Ovarian Tissue Cryopreservation Patient Flow

PDF icon 08b. Ovarian Tissue Transport Logistics

PDF icon 08c. Removing an Ovary for Fertility Preservation with Pathology Examination

Section 9. Administrative Forms

PDF icon 09a. Administrative Forms and Their Uses

File 09b. Form 1. Contact Information

Microsoft Office document icon 09d. Form 3. Request for Payment for Research Tissue Samples - UPDATE: The Oncofertility Consortium has reached its reimbursement cap.  No additional invoices will be processed. 

 

IMPORTANT NOTE: The Adverse Event Log is used for internal tracking of adverse events at your own institution.  This log may be shared with the Oncofertility Consortium (oncofertility@northwestern.edu) annually when you submit your IRB renewal documents but is not required.  The Serious Adverse Event Notification Form is used for internal tracking of serious adverse events at your own institution.  This log may be shared with the Oncofertility Consortium (oncofertility@northwestern.edu) at any time during the year for tracking purposes but is not required.  

Section 10. Laboratory Procedures and Forms

PDF icon 10a. Laboratory Procedure: Sample Labeling

PDF icon 10b. Ovarian Tissue Log

PDF icon 10c. Ovarian Tissue Cryopreservation Procedure

PDF icon 10d. Laboratory Procedure: Sample Labels

Other Resources

Training Video: Dissection of a Whole Ovary in Preparation for Cryopreservation

Training Video: Dissection of a Partial Ovary in Preparation for Cryopreservation