This Oncofertility Consortium resource is meant to function as a starter-kit so that researchers can establish the technique of alginate-based follicle culture in their own laboratories. Researchers should purchase their own reagents once they develop their techniques for follicle culture within their own labs. Any scientific products created as a result must attribute the grant number: NIH/NICHD P50HD076188 and the Oncofertility Consortium. Please also indicate if any issues arose during the process so we can work to mitigate these in the future.
For enhanced training, the OC offers a yearly Oncofertility 101 Course. For more information about Onco 101, please visit this link: http://oncofertility.northwestern.edu/oncofertility-101
Please refer to the Woodruff Lab Follicle Culture Handbook with Follicle Culture Take-Home Kit Addendum.
Anyone who wishes to receive a Follicle Culture Take-Home Kit must submit the following information via webform:
- Funding Source
- Project Significance, including background and any relevant preliminary data
- Project Summary, including specific aims and hypothesis
- Timeline for proposed studies
- NIH biosketch or CV
The kit will be shipped shortly after the application is reviewed and the requestor is notified. The requestor will pay all shipping costs associated with receiving the kit.
References for this work:
Tissue-engineered follicles produce live, fertile offspring. Xu M, Kreeger PK, Shea LD, Woodruff TK. Tissue Eng. 2006 Oct;12(10):2739-46.
Encapsulated three-dimensional culture supports development of nonhuman primate secondary follicles. Xu M, West-Farrell ER, Stouffer RL, Shea LD, Woodruff TK, Zelinski MB. Biol Reprod. 2009 Sep;81(3):587-94.
Methods for Follicle Isolation and Encapsulation: http://oncofertility.northwestern.edu/researchers/oncofertility-methods