Oncofertility Publications - Mammalian Folliculogenesis

It is critical to understand normal mammalian folliculogenesis to successfully recapitulate this process in vitro. Thus, a significant portion of research in the Division of Fertility Preservation is performed with the goal of understanding the basic biology of folliculogenesis as is evidenced in the following publications.

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Designing Follicle-Environment Interactions with Biomaterials (chapter 2)

Smith, R M., Woodruff T K., Shea L D. Cancer Treatment and Research. 2010; 156: 11-24. PMID 20811822.

 		Research Resources, Mammalian Folliculogenesis, Oncofertility-Related Publications
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The Structures that Underlie Normal Reproductive Function

Abstract

The mechanisms and physiology of reproductive function have fascinated scientists throughout time. Recent cellular and molecular level structural studies have provided unprecedented insights into reproductive systems and signaling networks. This 'cutting edge' editorial provides a recent example in each of these areas, namely, the anatomical integrity of the follicle, the molecular structure of activin with its binding partners and the molecular regulation of inhibin. These three examples of structure informing function help explain reproductive health and may provide solutions to reproductive disease.

Thomas F. Lerch, Min Xu, Theodore S. Jardetzky, Kelly E. Mayo, Ishwar Radhakrishnan, Ralph Kazer, Lonnie D. Shea, and Teresa K. Woodruff; Molecular Cell Endocrinol 267 1-5 2006

 		Basic Science, Research Resources, Mammalian Folliculogenesis, Oncofertility-Related Publications
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The Interactions Between the Stimulatory Effect of Follicle-Stimulating Hormone and the Inhibitory Effect of Estrogen on Mouse Primordial Folliculogenesis

Abstract

The murine primordial follicle pool develops largely within 3 days after birth through germline nest breakdown and enclosure of oocytes within pregranulosa cells. The mechanisms that trigger primordial follicle formation likely are influenced by a transition from the maternal to fetal hormonal milieu at the time of birth. High levels of maternal estrogen maintain intact germline nest in fetal ovary, and decrease of estrogen after birth is permissive of follicle formation. In the present study, we measured an increase in neonatal serum follicle-stimulating hormone (FSH), which corresponded to falling estradiol (E(2)) levels during the critical window of primordial follicle formation (Postnatal Days 1-3). To determine whether fetal hormones contribute in an active manner to primordial follicle formation, mouse fetal ovaries (17.5 days postcoitus) were cultured in vitro at two concentrations of E(2) (meant to reflect maternal and fetal levels of E(2)) and FSH for 6 days. High levels of E(2) (10(-6) M) inhibited germline nest breakdown, and this effect was significantly reduced when fetal ovaries were cultured in the low E(2) concentration (10(-10) M). FSH facilitated germline nest breakdown and primordial follicle formation under both high and low E(2) culture conditions. Low E(2) was identified as being more permissive for the effects of FSH on primordial follicle formation by stimulating the up-regulation of Fshr and activin beta A subunit (Inhba) expression, pregranulosa cell proliferation, and oocyte survival. The decrease of E(2) plus the presence of FSH after birth are critical for primordial follicle formation and the expression of oocyte-specific transcription factors (Figla and Nobox) in that inappropriate exposure to FSH or E(2) during follicle formation resulted in premature or delayed primordial folliculogenesis. In conclusion, with the drop of E(2) level after birth, FSH promotes primordial follicle formation in mice by stimulating local activin signaling pathways and the expression of oocyte-specific transcription factors.

Lei Lei, Shiying Jin, Kelly E. Mayo, and Teresa K. Woodruff. Biology of Reproduction, 82, 13-22 (2010).

 		Basic Science, Research Resources, Mammalian Folliculogenesis, Oncofertility-Related Publications
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Phylogenomic Analyses Reveal the Evolutionary Origin of the Inhibin a-Subunit, a Unique TGFb Superfamily Antagonist

Abstract

Transforming growth factor-beta (TGFbeta) homologues form a diverse superfamily that arose early in animal evolution and control cellular function through membrane-spanning, conserved serine-threonine kinases (RII and RI receptors). Activin and inhibin are related dimers within the TGFbeta superfamily that share a common beta-subunit. The evolution of the inhibin alpha-subunit created the only antagonist within the TGFbeta superfamily and the only member known to act as an endocrine hormone. This hormone introduced a new level of complexity and control to vertebrate reproductive function. The novel functions of the inhibin alpha-subunit appear to reflect specific insertion-deletion changes within the inhibin beta-subunit that occurred during evolution. Using phylogenomic analysis, we correlated specific insertions with the acquisition of distinct functions that underlie the phenotypic complexity of vertebrate reproductive processes. This phylogenomic approach presents a new way of understanding the structure-function relationships between inhibin, activin, and the larger TGFbeta superfamily.

Jie Zhu, Edward L. Braun, Satomi Kohno, Monica Antenos, Eugene Y. Xu, Robert W. Cook, S. Jack Lin, Brandon C. Moore, Louis J. Guillette, Jr., Theodore S. Jardetzky, Teresa K. Woodruff. PLoS One, Volume 5, Issue 3, March 2010.

 		Basic Science, Research Resources, Mammalian Folliculogenesis, Oncofertility-Related Publications
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The Role of the Extracellular Matrix in Ovarian Follicle Development

Abstract

Regulation of ovarian follicle development depends on endocrine- and paracrine-acting hormones, the 3-dimensional architecture of the follicle, and the physical rigidity of the surrounding tissue. These 3 forces are integrated throughout the life cycle of the follicle to ensure appropriate hormone secretion, differentiation of the somatic cells, and maturation of the oocyte. The process of in-follicle maturation provides a new tool for understanding ovarian follicle development under the influence of these factors.

Teresa K. Woodruff and Lonnie D. Shea; Reprod Sci. Vol 14 No 8 Suppl 6-10 Dec 2007

 		Basic Science, Research Resources, Mammalian Folliculogenesis, Oncofertility-Related Publications
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The Regulatory Role of Dicer In Folliculogenesis In Mice

Abstract

Dicer is the ribonuclease III for synthesis of mature functional microRNAs (miRNAs), which play an important role in regulating cell development. In the mouse ovary, the Dicer1 protein was expressed in both oocyte and granulosa cells of the follicle. In the present study, the role of miRNAs in mouse ovarian development was explored by using Dicer1 conditional knockout (cKO) mouse ovarian tissue (Amhr2 Cre/-; Dicer flox/flox), in which Dicer1 is deleted specifically in follicular granulosa cells. The morphology and gene expression profile of cKO and wild type (WT) mouse ovaries at various stages of development (day 4, day 8, 8 weeks and 8 months) were examined. Comparative analysis of the follicle number indicated that conditional inactivation of Dicer1 in the follicular granulosa cells led to an increased primordial follicle pool endowment, accelerated early follicle recruitment and more degenerate follicles in the cKO ovaries. In addition, significant differences were noted in the expression of some follicle development-related genes between cKO and WT mouse ovaries, such as Amh, Inhba, Cyp17a1, Cyp19a1, Zps, Gdf9 and Bmp15, suggesting the function of miRNAs in regulating gene expression is time- and gene-dependent. With the Dicer1 inactivation, mmu-mir-503, a miRNA that is more abundant in mouse ovary than in other tissues, was down-regulated significantly. Meanwhile, the expression of mmu-mir-503 decreased notably with follicle development in the gonadotropin-primed mouse ovary. Up-regulation of mmu-mir-503 in primary cultured granulosa cells resulted in the decreased expression of both the target gene and non-target gene at the transcriptional level, which involve genes related to granulosa cell proliferation and luteinization. In conclusion, Dicer1 plays important roles in follicular cell development through the differential regulation of gene expression.

Lei Lei, Shiying Jin, Gabriel Gonzalez, Richard R. Behringer, Teresa K. Woodruff; Mol Cell Endocrinol Epub ahead of print Sep 30 2009

 		Basic Science, Research Resources, Mammalian Folliculogenesis, Oncofertility-Related Publications
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Prepubertal Primordial Follicle Loss in Mice Is Not Due to Classical Apoptotic Pathways

Abstract

More than half of the primordial follicles that are formed by Day 6 of postnatal life in the mouse will be eliminated from the ovary by the time of puberty. Apoptosis, a form of programmed cell death, is one mechanism by which these follicles could be actively lost. To investigate whether apoptosis is responsible for the loss of primordial follicles, follicular atresia was examined during the prepubertal period, when follicles die and are cleared from the ovary at an extremely high rate. Four hallmarks of classical apoptosis were measured in follicles present in prepubertal ovaries. The primordial follicle cohort was not positively associated with nuclear condensation or cell shrinkage, activation of caspase 3, cleavage of poly(ADP ribose) polymerase 1 (PARP1), or fragmentation of DNA. These data are consistent with a nonapoptotic pathway that is responsible for small follicle death.

Candace M. Tingen, Sarah K. Bristol-Gould, Sarah E. Kiesewetter, Jason Tyler Wellington, Lonnie Shea, and Teresa K. Woodruff; Biology Reproduction Vol 81 No 1 16-25 July 2009

 		Basic Science, Research Resources, Mammalian Folliculogenesis, Oncofertility-Related Publications
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Extracellular Matrix Functions in Follicle Maturation

Abstract

The extracellular matrix (ECM) promotes and/or inhibits many cellular processes, including but not limited to proliferation, differentiation, and survival, which must occur for follicle growth and oocyte maturation. The ECM regulation of cellular processes in ovarian cells is being investigated in many animal models, including avian, rat, bovine, porcine, rabbit, sheep, human, and mouse. Granulosa cells are more frequently employed; however, the culture of intact follicles and ovaries has been developed and enables ECM functions in folliculogenesis to be studied. ECM components that have been examined are used individually (collagen, laminin, fibronectin) or collectively (Matrigel, isolated basal lamina, and ECM produced by cell lines) in both two- and three-dimensional model systems. In granulosa cell cultures, ECM affects morphology, aggregation and communication, survival, proliferation, and steroidogenesis; whereas follicle and ovary cultures demonstrate a regulation of folliculogenesis. This article describes the ECM functionality on ovarian cells throughout development, and highlights the potential of developing technologies to identify structure-function relationships in follicle maturation.

Courtney B. Berkholtz, Lonnie D. Shea, and Teresa K. Woodruff; Semin Reproducion Med Vol 24 No 4 262-9 Sep 2006

 		Basic Science, Research Resources, Mammalian Folliculogenesis, Oncofertility-Related Publications
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The Primordial Pool of Follicles and Nest Breakdown In Mammalian Ovaries

Abstract

The creation of the pool of follicles available for selection and ovulation is a multi-faceted, tightly regulated process that spans the period from embryonic development through to the first reproductive cycle of the organism. In mice, this development can occur in mere weeks, but in humans, it is sustained for years. Embryonic germ cell development involves the migration of primordial germs cells to the genital ridge, and the mitotic division of germ cell nuclei without complete cytokinesis to form a multi-nucleated syncytia, or germ cell nest. Through combined actions of germ cell apoptosis and somatic cell migration, the germ cell nuclei are packaged, with surrounding granulosa cells, into primordial follicles to form the initial follicle pool. Though often dismissed as quiescent and possibly uninteresting, this initial follicle pool is actually quite dynamic. In a very strictly controlled mechanism, a large portion of the initial primordial follicles formed is lost by atresia before cycling even begins. Remaining follicles can undergo alternate fates of continued dormancy or selection leading to follicular growth and differentiation. Together, the processes involved in the fate decisions of atresia, sustained dormancy, or activation carve out the follicle pool of puberty, the pool of available oocytes from which all future reproductive cycles of the female can choose. The formation of the initial and pubertal follicle pools can be predictably affected by exogenous treatment with hormones or molecules such as activin, demonstrating the ways the ovary controls the quality and quantity of germ cells maintained. Here, we review the biological processes involved in the formation of the initial follicle pool and the follicle pool of puberty, address the alternate models for regulating germ cell number and outline how the ovary quality-controls the germ cells produced.

Candace Tingen, Alison Kim, and Teresa K. Woodruff; Molecular Human Reproduction Vol. 15 No. 12 795-803 Dec 15, 2009

 		Basic Science, Research Resources, Mammalian Folliculogenesis, Oncofertility-Related Publications
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