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In vitro Growth Human Follicle with Antral Cavity
3D movie generated from a 100μm confocal z-stack (Zeiss LSM 510, 40x) of a human, 14-day in vitro growth follicle. This movie shows the newly formed antral cavity, the differentiation of a clear cumulus cell mass around the oocyte, and the mural granulosa cell layers. [F-Actin (red), Nuclei (blue)]. Movies were made on Volocity (Improvision/PerkinElmer).
Transzonal Projections in Immature in vivo Human Follicles
3D movie generated from a 100μm confocal z-stack (Zeiss LSM510, 40x) of a fresh-fixed, immature, human antral follicle showing the transzonal projections between the growing oocyte and the cumulus granulosa. [F-Actin (red), Nuclei (blue)].
